Technical resources
Practical guides for molecular biology work
Short, referenced articles on PCR, cloning, and handling research reagents. Written for planning and troubleshooting, not as a replacement for a validated protocol.
PCR & amplification · 8 min read
Taq vs Pfu: choosing a DNA polymerase for research PCR
A practical comparison of Taq and Pfu DNA polymerases: proofreading, error rate, extension speed, and when each enzyme is the better choice.
Published 2026-10-26
Cloning & assembly · 9 min read
Gibson Assembly troubleshooting: the failure points worth checking first
A practical checklist for failed Gibson Assembly reactions: overlap design, fragment quality, molar ratio, incubation, vector preparation, and transformation.
Published 2026-10-26
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Find the reagents discussed in these guides in the PCR and amplification reagents and DNA vectors and plasmids categories.